HUMAN LYMPHOCYTE TRANSFORMATION FOLLOWING INHIBITION OF PURINE NUCLEOSIDE PHOSPHORYLASE
نویسندگان
چکیده
منابع مشابه
Purine Nucleoside Phosphorylase
Purine nucleoside phosphorylase (EC 2.4.2.1) from bovine spleen is a trimeric enzyme that readily dissociates to the monomer. Dilution of enzyme from 20 to 0.02 pg of protein/ml is accompanied by a greater than 50-fold increase in the specific activity (utrimer = 0.23 nmol/min/pg; umonomer = 12.5 nmol/min/pg). Gel permeation chromatography in the presence of the substrate phosphate shows the en...
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Purine nucleoside phosphorylase has been purified about 7,300-fold and crystallized from human erythrocytes (mol wt 81,000). The recrystallized enzyme exists in the form of needles and sometimes bundles of needles and has a specific activity of 96 pM units per mg of protein. A number of phenomena reported earlier for a less pure preparation of this enzyme are still seen with the crystalline enz...
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The intracellular pathogen Toxoplasma gondii is a purine auxotroph that relies on purine salvage for proliferation. We have optimized T. gondii purine nucleoside phosphorylase (TgPNP) stability and crystallized TgPNP with phosphate and immucillin-H, a transition-state analogue that has high affinity for the enzyme. Immucillin-H bound to TgPNP with a dissociation constant of 370 pM, the highest ...
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INHIBITION AND STRUCTURE OF TOXOPLASMA GONDII PURINE 1 NUCLEOSIDE PHOSPHORYLASE 2 3 4 Running title: Toxoplasma gondii purine nucleoside phosphorylase 5 6 Teraya M. Donaldson, María B. Cassera, Meng-Chiao Ho, Chenyang Zhan, 7 Emilio F. Merino, Gary B. Evans, Peter C. Tyler, Steven C. Almo, Vern L. 8 Schramm, and Kami Kim d, # 9 10 From the Departments of Microbiology and Immunology, Biochemistr...
متن کاملRabbit Erythrocyte Purine Nucleoside Phosphorylase
1. Concave-downward double-reciprocal plots were obtained for rabbit erythrocyte purine nucleoside phosphorylase when the concentration of Pi was varied over a wide range at a fixed saturating concentration of either inosine or deoxyinosine. Similar behaviour was also displayed by the calf spleen enzyme. 2. The degree of curvature of double-reciprocal plots was greatly modified by the presence ...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 1977
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-197704000-00726